讲座题目: A Multi-Function Enzyme for the Repair of DNA Deamination

主讲人: Weiguo Cao, Professor of Department of Genetics and Biochemistry, Clemson University, South Carolina, USA.

主持人:李晓涛 教授

开始时间: 2015-7-10 14:00-15:30

讲座地址: 闵行生科院534报告厅

主办单位: 天美娱乐 科技处

报告人简介：Weiguo Cao is a Professor of Department of Genetics and Biochemistry, Clemson University. South Carolina, USA. Prof. Weiguo Cao awarded his PhD degree in 1992 in Microbiology, University of Idaho, Moscow, Idaho, USA. He was a postdoctoral fellow at Weill Cornell Medical College of Cornell University, New York, NY, USA (Formally Cornell University Medical College) from 1992 to 1997 and then he joined as Research Associate at Cornell University Medical College. In 2000 he was appointed as Assistant Professor at Department of Genetics and Biochemistry, Clemson University, Clemson, South Carolina, USA. He was promoted to Associate Professor in 2006 and professor in 2011. Prof. Weiguo Cao’s research interests are focused mainly on DNA damage, mutagenesis and repair; structure, function and evolution of repair enzymes, nitrosative stress and cellular response, nucleic acids amplification and detection, protein engineering.

报告摘要：Living organisms are exposed to highly reactive nitrogen species (RNS) from endogenous inflammation and in the environment as collectively defined as nitrogen oxides (NOx). Nitrosative stress imposed by exposure to RNS is a subclass of oxidative stress that leads to oxidative deamination in DNA. Adenosine is converted to inosine in DNA when it is exposed to nitrosating agents such as nitric oxide or nitrous acid. Endonuclease V (endo V) mediates an evolutionarily well-conserved pathway to repair adenosine deamination in both prokaryotic and eukaryotic organisms including mammals. Endo V is an enzyme that initiates repair of deaminated DNA bases by making an endonucleolytic incision at the 3’ side one nucleotide from a base lesion. The unusual biochemical mechanism of endo V-mediated lesion recognition, multiple endonuclease and exonuclease activities, and their implications in repair pathway will be presented in light of single-molecule, biochemical, genetic studies, as well as structural biological investigations. Endo V-mediated DNA repair is an important mechanism in maintaining genome integrity and preventing cancer in mammalian systems.